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目的观察培哚普利对去势大鼠骨质疏松的影响。方法将大鼠随机分为5组,每组10只。经切除大鼠双侧卵巢建立骨质疏松动物模型(OVX组)后,低(LP组)、中(MP组)、高剂量培哚普利组(HP组)经灌胃分别按2、4、8 mg/(kg·d)给药(溶解于1 ml生理盐水),正常对照组(CON组)仅切除卵巢周围部分脂肪组织,CON与OVX组仅灌胃等体积(1 ml)的生理盐水,均仅给药1次。给药24周后经股动脉采血并处死大鼠,检测血清钙、磷及碱性磷酸酶(ALP)活性,取股骨检测骨密度及生物力学变化。结果与CON组比较,OVX组血清钙、磷、ALP活性及骨密度明显下降(P<0.05)。与OVX组比较,MP及HP组大鼠的血清钙含量明显升高(P<0.05);MP组大鼠的血清磷含量明显升高(P<0.05);HP组大鼠的ALP活性明显升高(P<0.05),MP及HP组大鼠的骨密度均明显升高(P<0.05);MP组大鼠股骨的最大力学载荷明显升高(P<0.05)。结论各剂量培哚普利对改善骨质疏松模型大鼠的骨密度及生物力学均有效果,以中剂量培哚普利改善效果最佳。
Objective To observe the effect of perindopril on osteoporosis in ovariectomized rats. Methods The rats were randomly divided into 5 groups, 10 in each group. After the ovariectomized rats were established ovariectomized animal models of osteoporosis (OVX group), low (LP group), medium (MP group), high-dose perindopril group (HP group) , 8 mg / (kg · d) (dissolved in 1 ml of normal saline). In the normal control group (CON), only some of the adipose tissue around the ovary was excised. Saline, were given only once. Blood samples were taken from the femoral artery and sacrificed at 24 weeks after administration. Serum calcium, phosphorus and alkaline phosphatase (ALP) activity were measured. Bone mineral density (BMD) and biomechanical changes were measured. Results Compared with CON group, serum calcium, phosphorus, ALP activity and bone mineral density decreased significantly in OVX group (P <0.05). Compared with the OVX group, the serum calcium level in the MP and HP groups was significantly increased (P <0.05); the serum phosphorus level in the MP group was significantly increased (P <0.05); the ALP activity in the HP group was significantly increased (P <0.05). The BMD of MP and HP groups were significantly increased (P <0.05). The maximum mechanical load of femur in MP group was significantly increased (P <0.05). Conclusion Perindopril at various dosages can improve the bone mineral density and biomechanics in osteoporosis model rats.