论文部分内容阅读
目的:研究银杏叶提取物(EGB)对β-淀粉样蛋白(β-amyloid protein,Aβ)致阿尔茨海默病(Alzheimer’s disease,AD)模型大鼠学习记忆能力的影响及其作用机制。方法:用Y迷宫测定Aβ致AD大鼠学习记忆能力,苏木素-伊红(HE)染色,TUNEL法和免疫组化染色法分别检测其海马CA1区细胞形态学变化,神经元凋亡,Caspase-3P20的表达及Aβ的沉积,并观察EGB的保护作用。结果:Aβ致AD大鼠学习尝试次数明显增加,记忆正确次数明显减少;海马CA1区锥体细胞层损伤严重,可见到较多TUNEL和Caspase-3P20阳性神经元及Aβ阳性物质沉积。而银杏叶各剂量组均有不同程度的改善。结论:Aβ可引起β-淀粉样蛋白致AD大鼠海马CA1区神经元的凋亡,Caspase-3的激活参与了这一过程,而EGb有保护作用并能改善其学习记忆障碍。
Objective: To investigate the effects of Ginkgo biloba extract (EGB) on the learning and memory abilities of Alzheimer’s disease (AD) rats induced by β-amyloid protein (Aβ) and its mechanism. Methods: The learning and memory abilities of AD rats induced by Aβ were determined by Y maze. The changes of hippocampal CA1 cell morphology, neuronal apoptosis, Caspase-3 expression were detected by hematoxylin-eosin (HE) staining, TUNEL staining and immunohistochemistry. 3P20 expression and Aβ deposition, and observe the protective effect of EGB. Results: The number of learning and trying times of Aβ-induced AD rats increased significantly, and the correct times of memory were significantly reduced. The damage of pyramidal cell layer in hippocampal CA1 region was serious. More TUNEL, Caspase-3P20 positive neurons and Aβ positive neurons were found. The ginkgo leaf dose groups have different degrees of improvement. CONCLUSION: Aβ can induce the apoptosis of hippocampal CA1 neurons induced by β-amyloid in AD rats. Caspase-3 activation is involved in this process, while EGb has protective effect and can improve learning and memory impairment.