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目的:探讨类风湿关节炎(rheumatoid arthritis,RA)患者骨代谢相关基因骨保护素、核因子κB受体活化因子配体(RANKL)及核转录因子κB(NF-κB)、骨形态发生蛋白(BMP)信号通路的表达。方法:应用聚合酶链式反应(PCR)技术检测40例RA患者和32例健康对照者外周血单个核细胞(PBMC)中骨保护素、RANKL、NF-κB p50、NF-κB p52、NF-κB p65、BMP-2、BMP-9的mRNA表达水平,比较两组骨保护素/RANKL比值,并分析差异有统计学意义的指标与红细胞沉降率(ESR)、血清C-反应蛋白(CRP)和DAS28评分等疾病活动指标的相关性。另选取10例初发RA患者和10例健康对照者,检测其PBMC中NF-κB p65 mRNA的水平。结果:与健康对照者相比,RA患者RANKL mRNA相对表达量明显上升(P<0.01),骨保护素/RANKL比值明显下降(P<0.01),NF-κB p65 mRNA相对表达量明显下降(P<0.01),骨保护素、NF-κB p50、NF-κB p52、BMP-2及BMP-9的mRNA相对表达量两组间差异无统计学意义,RANKL、NF-κB p65、骨保护素/RANKL与血ESR、血清CRP和DAS28评分无相关性。与健康对照组相比,初发RA患者的NF-κB p65 mRNA表达量增高(P<0.05)。结论:RA患者PBMC表达破骨指标增加,并可能通过NF-κB信号通路影响骨代谢。
OBJECTIVE: To investigate the effects of osteoprotegerin, RANKL, NF-κB and Bone Morphogenetic Proteins (BMPs) in patients with rheumatoid arthritis (RA) BMP) signaling pathway expression. Methods: The expressions of osteoprotegerin, RANKL, NF-κB p50, NF-κB p52, NF-κB p50 in peripheral blood mononuclear cells (PBMCs) from 40 RA patients and 32 healthy controls were detected by polymerase chain reaction (PCR) The levels of osteoprotegerin / RANKL were compared between the two groups. The differences of the indexes of erythrocyte sedimentation rate (ESR), serum C-reactive protein (CRP) And DAS28 score and other indicators of disease activity. Another 10 patients with primary RA and 10 healthy controls were selected to detect the level of NF-κB p65 mRNA in PBMCs. Results: Compared with healthy controls, the relative expression of RANKL mRNA in RA patients was significantly increased (P <0.01), the ratio of osteoprotegerin / RANKL was significantly decreased (P <0.01), while the relative expression of NF-κB p65 mRNA was significantly decreased 0.01). There was no significant difference in the relative expression of osteoprotegerin, NF-κB p50, NF-κB p52, BMP-2 and BMP-9 between the two groups. The expressions of RANKL, NF- RANKL had no correlation with blood ESR, serum CRP and DAS28 scores. Compared with the healthy control group, the expression of NF-κB p65 mRNA in newly diagnosed RA patients increased (P <0.05). Conclusion: The expression of osteoclast of PBMC in RA patients increases, and may affect bone metabolism through NF-κB signaling pathway.