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目的:研究表达Angiostatin基因的B16黑色素瘤的体内生长行为.方法:通过改造Plasminogen cDNA得到Angiostatin基因,构建成真核表达载体pAGAGS3后导入B16黑色素瘤细胞使其表达.结果:表达Angiostatin的B16黑色素瘤细胞体外生长速率和软琼脂中克隆形成能力均未改变.但接种小鼠26d后,体内生长抑制率达87%(P<0.01).结论:表达Angiostatin的B16黑色素瘤体内生长受到显著抑制.
Objective: To study the in vivo growth behavior of B16 melanoma expressing Angiostatin gene. Methods: Angiostatin gene was obtained by transforming Plasminogen cDNA to construct an eukaryotic expression vector pAGAGS3 and then introduced into B16 melanoma cells for expression. Results: B16 melanoma expressing Angiostatin The growth rate of cells in vitro and the ability of colony formation in soft agar did not change. However, after 26 days of inoculation, the growth inhibition rate in vivo reached 87% (P<0.01). Conclusion: The growth of B16 melanoma expressing angiostatin is significantly inhibited in vivo.