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目的:为了探讨在生产条件下采用哪种方法来检测抗绿脓杆菌外毒素-A血浆(马)的抗体效价更为可行.方法:该文对小鼠中和试验,双向琼脂扩散试验及胶乳凝集试验进行了比较研究.结果实验显示,小鼠中和试验和双向琼脂扩散试验特异性良好,结果稳定,但前者灵敏度比后者更高.而胶乳凝集试验由于胶乳抗原不易保存,其结果稳定性较差.结论:我们认为小鼠中和试验适用于大规模生产时采用,双向琼脂扩散可作为初步效价的检测.“,”In order to approach which method is the most suitable one in the titre detection of equine anti-Pseudomonas areruginosa exotoxin A plasma. METHOD:Mouse neutralization test. Double immunodiffusion test and latex agglutination reaction were compared RESULTS:The results showed that the mouse neutralization test and double immunodiffusion test have the advantages of good-special and reliable-resuirs,but sensitivity of the former is higher than one of the latter. The results from latex agglutination were unreliable due to latex anigen was preserved uneasy. CONCLUSION: So we sugest that the mouse netralization was best suited for detecting the titre,double immunodiffusion test was available.