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目的系统了解罗勒Ocimum basilicum花和叶的转录组特征,丰富罗勒转录组数据信息。方法选取罗勒新鲜花朵和叶片为材料,利用Illumina Hi SeqTM 2500进行高通量测序,构建罗勒花和叶转录组文库,并用测序评估、转录组数据组装和基因功能注释等生物信息学方法进行分析。结果对原始数据进行除杂之后,共获得86 331 137个reads片段,包含了6 455 365 309个核苷酸序列信息;将质控后得到的高质量序列进行de novo拼接,得到了90 341个Unigene片段。将Unigene与COG数据库进行比对表明,罗勒的花和叶转录组中的Unigene根据功能可大致分为25类;根据GO功能分类可大致分为生物过程、细胞组分和分子功能3大类43分支;利用KEGG数据库作为参考,依据代谢通路可以将转录组中的数据分成111类,包括生化代谢通路、植物病原体互作、DNA剪切、植物激素生物合成、苯丙氨酸生物合成、萜类化合物与类固醇类化合物合成、脂类代谢、RNA降解等。MISA软件成功设计15 617对SSR引物,检测到10 254个SNP位点。结论研究结果为后期罗勒功能基因的挖掘、代谢途径及调控机制的研究奠定了理论基础。
Objective To understand the transcriptome characteristics of flowers and leaves of Ocimum basilicum and to enrich the data of basil transcriptome. Methods Fresh flowers and leaves from basil were selected for high throughput sequencing with Illumina Hi SeqTM 2500. The library was constructed from basil leaves and leaves and analyzed by bioinformatics methods such as sequencing, data assembly and gene function annotation. Results A total of 86 331 137 reads including 6 455 365 309 nucleotide sequence information were obtained after the original data was denuded. The high quality sequences obtained after quality control were de novo spliced to obtain 90 341 Unigene fragment. The alignment of Unigene with the COG database showed that Unigene in flower and leaf transcriptomes of basil can be divided into 25 categories according to their functions. According to the classification of GO functions, they can be divided into three categories: biological processes, cellular components and molecular functions Based on the KEGG database, the data in the transcriptome can be divided into 111 categories based on the metabolic pathway, including biochemical metabolic pathways, plant pathogen interactions, DNA shearing, plant hormone biosynthesis, phenylalanine biosynthesis, terpenoids Synthesis of compounds and steroids, lipid metabolism, RNA degradation and so on. 15 617 pairs of SSR primers were successfully designed by MISA software and 10 254 SNPs were detected. Conclusion The results of this study laid the theoretical foundation for the study of the basal mineralization, metabolic pathway and regulation mechanism of late basal function genes.