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目的:观察参麦注射液对白细胞介素-1β(IL-1β)体外诱导兔软骨细胞增殖的影响。方法:采用IL-1β体外诱导兔软骨细胞的方法,建立变性软骨细胞模型,将其分为空白组、模型组、1%参麦组、5%参麦组和10%参麦组。空白组采用含10%胎牛血清的DMEM培养液培养;模型组采用含10ng/mL IL-1β的DMEM培养液培养;1%、5%、10%参麦组采用含10ng/mL IL-1βDMEM培养液培养24h,再分别采用含1%、5%、10%参麦注射液的DMEM培养液培养。采用MTT比色法检测软骨细胞的增殖情况。结果:模型组吸光度值(OD值)低于空白组,2组比较,差异有非常显著性意义(P<0.01),说明IL-1β可抑制软骨细胞的增殖,建立变性软骨细胞模型。模型组及不同浓度参麦组间的OD值比较,差异均有非常显著性意义(P<0.01),不同浓度的参麦注射液均可明显促进变性软骨细胞的增殖,其增殖作用与参麦注射液浓度呈正相关。结论:参麦注射液能够明显促进IL-1β体外诱导兔软骨细胞的增殖,这可能是参麦注射液防治关节退变的机理之一。
Objective: To observe the effect of Shenmai injection on rabbit chondrocyte proliferation induced by interleukin-1β (IL-1β) in vitro. Methods: Rabbit chondrocytes were induced by IL-1β in vitro. The chondrocytes were divided into blank group, model group, 1% Shenmai group, 5% Shenmai group and 10% Shenmai group. The blank group was cultured in DMEM medium containing 10% fetal bovine serum. The model group was cultured in DMEM medium containing 10ng / mL IL-1β; the medium containing 10ng / mL IL-1βDMEM at 1%, 5%, 10% The culture medium was cultured for 24h, and then cultured in DMEM medium containing 1%, 5% and 10% Shenmai injection respectively. The proliferation of chondrocytes was detected by MTT colorimetry. Results: The OD value of the model group was lower than that of the blank group. There was a significant difference between the two groups (P <0.01), indicating that IL-1β could inhibit the proliferation of chondrocytes and establish a degeneration chondrocyte model. There was significant difference in OD value between model group and different concentrations of Shenmai group (P <0.01). Different concentrations of Shenmai injection could obviously promote the proliferation of degenerative chondrocytes, Injection concentration was positively correlated. Conclusion: Shenmai injection can significantly promote the proliferation of rabbit chondrocytes induced by IL-1β in vitro, which may be one of the mechanisms of Shenmai injection in preventing and treating joint degeneration.