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目的:研究大鼠肠道水解酶(LPH酶)对射干黄酮苷中射干苷及野鸢尾苷大鼠肠道吸收特征的影响。方法:应用大鼠在体肠灌流模型,采用高效液相法测定射干苷及野鸢尾苷含量,比较加入LPH酶抑制剂葡糖酸内酯前后,射干苷、野鸢尾苷肠道吸收率变化。结果:与未加入抑制剂前比较,2个射干黄酮苷成分在大鼠十二指肠、空肠、回肠、结肠的水解抑制率均有不同程度的变化,其中射干苷在4个肠段的水解抑制率分别为66%、52%、43%、38%;野鸢尾苷分别为51%、45%、26%、31%。结论:LPH酶抑制剂葡糖酸内酯能一定程度地抑制射干苷和野鸢尾苷的水解,提示大鼠肠黏膜上的LPH酶可能参与了射干中射干苷和野鸢尾苷在肠道的代谢过程。
OBJECTIVE: To study the effect of rat intestinal hydrolase (LPHase) on the intestinal absorption characteristics of dry glycosides and wild iriside in rats. Methods: Rat intestinal perfusion model was established. The content of canthaxanthin and silymarin were determined by HPLC. The intestinal absorption rate of canthaxanthin and irisoside were compared before and after adding LPH inhibitor gluconolactone. Results: Compared with those before the inhibitor, the inhibitory rates of two Chinese medicinal flavonoid glycosides in rat duodenum, jejunum, ileum and colon were all changed to different degrees. Among them, The inhibitory rates were 66%, 52%, 43% and 38%, respectively. The wild irises were 51%, 45%, 26% and 31%, respectively. CONCLUSION: LPH enzyme inhibitor gluconolactone can inhibit the hydrolysis of spiced glycosides and wild tectori glycosides to a certain extent, suggesting that the LPH enzyme in the intestinal mucosa of rats may be involved in the metabolism of emodin and stilbene in the gut process.