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对海岛棉产量和早熟性状进行QTL初步定位,为分子标记辅助育种提供依据。利用5200多对SSR引物筛选海岛棉品种新海3号和Giza82间的多态性引物,获得107对。以多态性引物检测新海3号×Giza82的190个F2:3家系,获得120个多态性位点。利用JoinMap3.0分析软件构建了一个包含22个连锁群,74个标记,标记间平均距离12.06 cM,全长893 cM,覆盖海岛棉基因组20.12%的分子标记遗传连锁图谱。采用复合区间作图法检测到21个与海岛棉产量性状和早熟性状有关的QTL,其中早熟性状检测到12个QTL,分别位于1、3、5、6、11、17、22共7个连锁群上;产量性状检测到9个QTL,分别位于1、4、5、6、7、16、22共7个连锁群上。研究结果为海岛棉产量性状和早熟性状的分子设计育种提供了有用的信息。
QTL mapping of the yield and precocity traits of island cotton provided the basis for molecular marker-assisted breeding. A total of 107 polymorphic primers were screened from more than 5200 pairs of SSR primers in Xinhai 3 and Giza82. Polymorphic primers were used to detect 190 F2: 3 families in Xinhai 3 × Giza82, and 120 polymorphic loci were obtained. Using JoinMap3.0 software, a molecular marker genetic linkage map consisting of 22 linkage groups, 74 markers with an average distance of 12.06 cM, a total length of 893 cM and a coverage of 20.12% of G. barbadense genome was constructed. Twenty-one QTLs related to yield traits and precocious traits of G. barbadense were detected by composite interval mapping. Among them, 12 QTLs were detected in precocious traits, which were located in 7, 1, 3, 5, 6, 11, 17, Nine QTLs were detected in yield traits, which were located in 7 linkage groups of 1, 4, 5, 6, 7, 16, and 22, respectively. The results provided useful information for the molecular design and breeding of yield traits and prematurity traits in island cotton.