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目的:建立黄连4种不同炮制品的特征图谱,探讨饮片炮制前后化学成分的变化规律,并采用HPLC法对其中木兰碱、药根碱、黄连碱、巴马汀、小檗碱5个生物碱类成分进行定量分析比较。方法:采用Kromasil-C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-50 mmol.L-1磷酸二氢钾溶液(磷酸调pH 3.0)(45∶55),内含十二烷基硫酸钠12.5 mmol.L-1;检测波长275 nm;流速1 mL.min-1;柱温为30℃;进样量10μL。结果:所建立的特征图谱信息丰富,5个生物碱成分在测定范围内线性关系较好(r>0.9994),平均回收率分别为102.8%,96.9%,98.4%,104.8%,103.7%。结论:初步实验表明,黄连不同炮制品的特征图谱有所差别,相关成分在炮制前后发生了量变和质变,并呈现一定的变化规律。
OBJECTIVE: To establish the characteristic maps of four different processed products of Coptis chinensis Franch.METHODS, to investigate the chemical composition changes of the processed pieces before and after processing, and to determine the chemical constituents of the four alkaloids of Magnolia bark, jatrorrhizine, berberine, berberine, Quantitative analysis and comparison of class components. Methods: Kromasil-C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was acetonitrile-50 mmol·L-1 potassium dihydrogen phosphate solution (adjusted to pH 3.0 with phosphoric acid) (45:55) Sodium sulfite 12.5 mmol.L-1; detection wavelength 275 nm; flow rate 1 mL.min-1; column temperature 30 ℃; injection volume 10μL. Results: The established characteristic maps were abundant. The five alkaloids showed a good linear relationship (r> 0.9994) with the average recoveries of 102.8%, 96.9%, 98.4%, 104.8% and 103.7%, respectively. Conclusion: The preliminary experiment shows that the characteristic patterns of different processed products of Coptis chinensis differ. The related components have been changed and changed qualitatively before and after the processing, showing a certain variation pattern.