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１９９６～１９９７年用柯赫法则对套袋鸭梨黑点病进行了病原诊断，初步明确该病是由细交链孢菌（ＡｌｔｅｒｎａｒｉａｔｅｎｕｉｓＮｅｅｓ）和粉红单端孢菌（ＴｒｉｃｈｏｔｈｅｃｉｕｒｍｒｏｓｅｕｒｎＬｉｎｋ）真菌侵染所致。两种菌在ＰＤ培养液中振荡培养２１天，过滤，高压灭菌获得毒性培养液（毒素粗提液），粗毒素处理梨果出现典型的黑点病症状。
From 1996 to 1997, Koch’s rule was used to diagnose the bagged pear black spot disease. It was initially determined that the disease was caused by the fungus Alternaria nees and TrichotheciurmroseurnLink. The two kinds of bacteria were cultured in PD medium for 21 days under shaking, and the toxic medium (toxin crude extract) was obtained by filtration and autoclaving. The typical symptoms of black spot were observed in crude toxin treatment.