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以１：１０兔抗猪脊髓前角运动神经元血清（ｒａｂｂｉｔａｎｔｉ－ｓｗｉｎｅｍｏｔｏｎｅｕｒｏｎｓｅｒｕｍ，ＲＡＳ）、正常兔血清（ｎｏｒｍａｌｒａｂｂｉｔｓｅｒｕｍ，ＮＲＳ）、肌肉提出物（ｍｕｓｃｌｅｅｘｔｒａｃｔｓ，ＭＥＴ，５０和１００μｇ／ｍｌ）、脑提出物（ｂｒａｉｎｅｘｔｒａｃｔｓ，ＢＥＴ，５０和１００μｇ／ｍｌ）分别作用于体外培养的新生大鼠脊髓组织块，５ｄ后观察神经突起的生长情况。结果表明，ＲＡＳ可明显抑制大鼠脊髓组织块生长（ＮＲＳ组有突起生长的脊髓块占３６．７％，ＲＡＳ组仅为１３．３％），而ＭＥＴ、ＢＥＴ均可明显促进大鼠脊髓组织块生长（ＭＥＴ及ＢＥＴ组有突起生长的脊髓块均占９６．７％）。同时比较ＲＡＳ对猪、人和大鼠脊髓的免疫组织化学交叉反应，证明ＲＡＳ对运动神经元（Ｍｎｓ）的免疫反应与动物的种属关系不大。
Rabbit anti-swinemotoneuronserum (RAS), normalrabbit serum (NRS), muscleextracts (MET, 50 and 100μg / ml) brainextracts, BET, 50 and 100μg / ml) were used to treat the spinal cord tissue of neonatal rats in vitro. The neurite growth was observed 5 days later. The results showed that RAS significantly inhibited the growth of spinal cord tissue in rats (36.7% of the spinal cord masses in the NRS group and only 13.3% in the RAS group), while MET and BET significantly promoted the spinal cord tissue Block growth (MET and BET group have protruding growth of the spinal cord blocks accounted for 96.7%). At the same time, the immunohistochemical cross-reaction of RAS on the spinal cord of pig, human and rat was also compared. It was proved that the immune response of RAS to motor neurons (Mns) was not related to the species of animals.