取110例开胸手术切下造血功能正常的肋骨,挤出骨髓作粒-巨噬祖细胞(GM-CFU-C)的体外琼脂培养。在琼脂板上形成的集落数范围为33.4～571.6个/2×10~5有核细胞,平均值为164.0±10.4个/2×10~5有核细胞,形成75个以上细胞集落数人数占110例的77.3％。分析研究了影响体外琼脂培养效果的主要因素,其中包括有核细胞的分离浓缩、稀释计数方法、血清的使用等。 110 cases of thoracotomy were performed to cut the normal hematopoietic ribs, and the bone marrow was extruded for granulocyte-macrophage progenitor cells (GM-CFU-C) in vitro agar. The number of colonies formed on agar plates ranged from 33.4 to 571.6 cells / 2 × 10 ~ 5 nucleated cells, with an average of 164.0 ± 10.4 cells / 2 × 10 ~ 5 nucleated cells, accounting for more than 75 cell colonies 77.3% of 110 cases. Analyzed and studied the main factors affecting the effect of agar culture in vitro, including the separation and concentration of nucleated cells, dilution counting method, the use of serum and so on.