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采用38种培养基,173份基因型材料。4年大量反复试验表明:甜菜未授粉胚珠接种在附加单一激素(6BA或K)或激素组合(IAA+K)或(NAA+6BA)的PGOB、MS、1/2MS、改良MS或N6基本培养基上.均可成功诱导单倍体植珠。胚囊发育至成熟阶段的胚珠诱导效果为佳。胚珠植株转移至附加IBA、NAA、IBA+GA、IAA+K或IAA的MS培养基上可诱导生根。分化、扩繁、壮苗培养基为MS基本培养基附加K和IAA。在培养基中附加0.01％秋水仙碱处理1—2天,可使单倍体植株染色体加倍率达66.0％。细胞学观察表明:单倍体胚状体起源为卵细胞或反足细胞。后代遗传学观察表明:同一诱导材料的不同胚珠株系表现出多样性,同一株系个体间性状整齐一致.表现出高度纯合性。胚珠再生植株后代含糖率明显较高,且个体间差异小。
38 media, 173 genotypes were used. Extensive trials over 4 years showed that the untested beets of sugar beet were inoculated with PGOB, MS, ½ MS, modified MS or N6 basal culture with single hormone (6BA or K) or hormonal combination (IAA + K) or (NAA + 6BA) Based on the successful induction of haploid pearls. Ovule induction effect of embryo sac to mature stage is better. Ovule plants can be induced to root on MS medium supplemented with IBA, NAA, IBA + GA, IAA + K or IAA. Differentiation, propagation, seedling medium for MS basic medium attached to K and IAA. In the medium supplemented with 0.01% colchicine for 1-2 days, haploid chromosome doubling rate of 66.0%. Cytological observations showed that the haploid embryoid body originated from oocytes or antipodal cells. The progeny genetics showed that the different ovules of the same inducible material showed diversity, and the homozygous traits in the same strain were homogeneity and showed a high degree of homozygosity. The ovules of ovules regenerated plants had significantly higher sugar content, and the difference between individuals was small.