论文部分内容阅读
采用形态学和现代分子生物学方法,对河南省豫西地区农田土壤镰孢菌种群多样性进行了分析。通过鉴定,共得到了9个种的镰孢菌,分别为尖孢镰孢菌、半裸镰孢菌、轮枝镰孢菌、禾谷镰孢菌、层生镰孢菌、锐顶镰孢菌、黄色镰孢菌、木贼镰孢菌和茄腐镰孢菌。系统发育学分析结果表明:镰孢菌属真菌18S rDNA-ITS和EF-1α基因序列均具有一定的同源性和丰富的遗传多态性,其中李瑟组镰孢菌轮枝镰孢菌、层生镰孢菌、黄色镰孢菌种间18S rDNA-ITS基因序列存在着较高的同源性,不能正确地表现镰孢菌属种一级分类单元的特征;而延伸因子EF-1α基因序列却较好地表现出了供试菌株种一级的关系,说明镰孢菌延伸因子EF-1α基因序列较18S rDNA-ITS基因序列存在更丰富的种间多态性,可以较好地表现镰孢菌种一级的关系。
Morphological and modern methods of molecular biology were used to analyze the diversity of soil fusarium species in farmland in western Henan province. Through the identification, a total of nine species of Fusarium were obtained, which were Fusarium oxysporum, Fusarium moniliforme, Fusarium moniliforme, Fusarium graminearum, Fusarium venenatum, Fusarium verticillum Fusarium yellow, Fusarium equisetifolia and Fusarium solani. Phylogenetic analysis showed that the 18S rDNA-ITS and EF-1α gene sequences of Fusarium fungi had some homologies and abundant genetic polymorphisms. Fusarium verticillioides Fusarium venenatum, The 18S rDNA-ITS gene sequence of Fusarium venenatum and Fusarium yellow feldspar showed high homology and could not correctly represent the characteristics of the first taxonomic unit of Fusarium species. The elongation factor EF-1α gene The results showed that the Fusarium elongation factor EF-1α gene sequence had a richer interspecific polymorphism than the 18S rDNA-ITS gene sequence, Fusarium species a relationship.