论文部分内容阅读
目的评价卵清蛋白(OVA)饲喂致敏OVA特异性T细胞受体转基因小鼠(OVA23-3)的方法建立过敏性肠炎模型的可行性,并观察其免疫学指标变化。方法采用高OVA饲料饲喂OVA23-3小鼠的方法,建立小鼠过敏性肠炎模型。通过ELISA法检测血清OVA特异性IgE抗体和细胞培养上清细胞因子的含量,流式细胞术检测细胞内细胞因子。结果高OVA饲料饲喂引起OVA23-3小鼠体重下降,并发生以小肠为主的出血性炎症。与对照组比较,实验组血清OVA特异性IgE含量明显增高,细胞培养上清IL-4水平增高,而IFN-γ水平降低,同时CD4+T细胞中IL-4产生细胞百分率显著增加,IFN-γ产生细胞百分率显著减少。结论高OVA饲料饲喂OVA23-3小鼠诱发过敏性肠炎的方法是一种理想快速简便的方法,Th2型细胞反应占优势是其重要特征。
Objective To evaluate the feasibility of ovalbumin (OVA) feeding sensitized OVA-specific T cell receptor transgenic mice (OVA23-3) to establish an allergic enteritis model and observe the changes of immunological indexes. Methods OVA23-3 mice were fed with high OVA diet to establish mouse model of allergic enteritis. Serum OVA specific IgE antibody and cell culture supernatants were detected by ELISA. Cytokines were detected by flow cytometry. Results High OVA diet resulted in weight loss in OVA23-3 mice and hemorrhagic inflammation mainly in the small intestine. Compared with the control group, the level of serum IgA-specific IgE in the experimental group was significantly increased, the level of IL-4 in the cell culture supernatant was increased, and the level of IFN-γ was decreased. Meanwhile, the percentage of IL-4-producing cells in CD4 + T cells was significantly increased, γ-producing cells significantly reduced. Conclusions The method of hypersensitive colitis induced by high OVA diet in OVA23-3 mice is an ideal and rapid method. Th2 type cytokines predominate is an important feature.