研究了苄基三甲基溴化铵动态改性、十四烷基吡啶盐等“永久性”涂渍的两种反相色谱,系统分离和间接光度检测了强极性、弱紫外吸收羟基酸。测定这些检测和改性试剂在YWG-CH反相填料和甲醇-磷酸缓冲液色谱体系的吸附等温线,探讨试剂在固定相的吸附量和其它因素对羟基酸保留、检测灵敏度的影响及分离检测机理。 Two kinds of reversed phase chromatography, “permanent” coating such as benzyltrimethylammonium bromide dynamic modification and tetradecylpyridinium salt, were systematically studied. The strong polar and weak ultraviolet absorbing hydroxy acids . The adsorption isotherms of these detection and modification reagents in YWG-CH reversed-phase media and methanol-phosphoric acid buffer chromatography system were investigated. The effects of adsorption amount of the reagent in the stationary phase and other factors on the retention of hydroxyl acid and detection sensitivity were discussed. mechanism.