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本文用图像分析仪对叮咬经不同抗原免疫接种兔后的中华硬蜱中肠消化细胞ＤＮＡ进行测量分析。结果表明：蜱血餐后其中肠消化细胞ＤＮＡ增加，约第５天达高峰。佐剂对照组吸血后蜱消化细胞ＤＮＡ倍体数显著增大，第５天ＤＮＡ平均倍体数可达６８Ｃ，峰值达１１８Ｃ，≥５Ｃ细胞达３４％。５天后所有消化细胞均≥５Ｃ。中华硬蜱叮咬不同抗原免疫接种兔后中肠消化细胞ＤＮＡ量血餐后不能有效地增加。影响程度依次为柱层析１０５ＫＤ纯化抗原组、中肠抗原组、唾液腺抗原组、全虫抗原组和卵抗原接种组。除卵抗原组外，其它各免疫组平均ＤＮＡ均＜５Ｃ，≥５Ｃ的细胞数＜１７％，峰值＜７Ｃ，且各时期均可见数量不等的２Ｃ和３－４Ｃ细胞，与对照组比较均有显著性差异（Ｐ＜００５）。上述结果提示相关的抗原免疫接种宿主后，可使宿主产生获得性免疫力，导致中华硬蜱吸血后中肠消化细胞ＤＮＡ在血液消化过程中的正常增加受到严重影响，其机理可能与ＤＮＡ合成受阻及消化细胞破坏有关。本实验的五个免疫接种组中以纯化抗原接种组作用最强，在选择制作抗蜱疫苗时可供参考。
In this paper, image analysis instrument was used to measure the digestive cell DNA in the midgut of Ixodes sinensis after bite rabbits immunized with different antigens. The results showed that the amount of digested cell DNA increased after the meal blood meal reached its peak on the 5th day. The DNA ploidy of digested cells in the adjuvant-treated group increased significantly after ingestion. On the fifth day, the average number of ploidy DNA reached 68C, the peak value reached 118C, and the percentage of ≥5C cells reached 34%. All digested cells were> 5C after 5 days. Chinese Ixodes bite different antigen immunized rabbits after midgut digestion cell DNA amount of blood meal can not be effectively increased. The influence degree followed by column chromatography 105KD purified antigen group, midgut antigen group, salivary gland antigen group, all insect antigen group and egg antigen inoculation group. In addition to the egg antigen group, the average DNA of other immunization groups were <5C, the number of cells ≥ 5C <17%, the peak <7C, and the number of 2C and 3-4C cells ranging from different age There was a significant difference (P <0 05). The above results suggest that after the host immunized with the relevant antigen, the host can produce adaptive immunity, leading to the normal increase of digestive cell DNA in midgut digestion during the process of blood digestion, which may be due to DNA synthesis inhibition And digestive cell damage related. In this experiment, the five vaccinated groups with the strongest effect of the purified antigen inoculation group, choose to make anti-tick vaccine for reference.