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目的 研究亚硒酸钠对苯并 [a]芘 (BaP)诱导的小鼠肺细胞DNA损伤的影响。方法 选用雄性昆明种小鼠。亚硒酸钠采用腹腔注射处理 ,BaP灌胃染毒。BaP单独作用的剂量是 12 5、2 5 0、5 0 0mg/kg;硒和BaP联合作用组采用 0 .75、1.5 0、3.0 0mg/kg的亚硒酸钠分别与 2 5 0mg/kgBaP联合染毒。设立溶剂对照组。用单细胞凝胶电泳方法检测DNA损伤的情况。结果 12 5、2 5 0、5 0 0mg/kgBaP组小鼠肺细胞DNA损伤程度较对照组严重 ,差异有显著性 (P <0 .0 1) ,总损伤细胞百分率分别为4 3.5 0 %、84 .0 0 %、95 .6 3% ,较对照组 (9.75 % )明显增高 ,差异亦有显著性 (P <0 .0 1) ,且存在着明显的剂量 -反应关系。 0 .75、1.5 0、3.0 0mg/kg的亚硒酸钠对 2 5 0mg/kgBaP诱导的小鼠肺细胞DNA损伤具有明显的拮抗效应 ,1.5 0mg/kg亚硒酸钠的拮抗作用优于 0 .75、3.0 0mg/kg的亚硒酸钠 ,差异有显著性 (P <0 .0 5 )。结论 12 5～ 5 0 0mg/kg的BaP可以引起小鼠肺细胞DNA损伤 ,而 0 .75～ 3.0 0mg/kg的亚硒酸钠对 2 5 0mg/kgBaP诱导的小鼠肺细胞DNA损伤具有明显的拮抗作用。
Objective To study the effect of sodium selenite on DNA damage in lung cells induced by benzo [a] pyrene (BaP) in mice. Methods Male Kunming mice were selected. Sodium selenite by intraperitoneal injection, BaP intragastric administration. The dosage of BaP alone was 12 5, 25 0, 500 mg / kg; the combination of selenium and BaP was treated with 0 .75, 1.5 0, 3.0 0 mg / kg sodium selenite and 250 mg / kg Ba Toxicity. Set up solvent control group. Single cell gel electrophoresis was used to detect DNA damage. Results The DNA damage of lung cells in 12, 25, 500 mg / kg BaP group was more severe than that in control group (P <0.01), and the percentage of total damaged cells was 43.5% 84.0%, 95.63%, which was significantly higher than that of the control group (9.75%), the difference was also significant (P <0.01), and there was a significant dose-response relationship. 0. 75,1.5 0,3.0 0 mg / kg of sodium selenite on 250 mg / kg BaP induced DNA damage in lung cells had a significant antagonistic effect, 1.5 0mg / kg of sodium selenite antagonistic effect is better than 0 .75,3.0 0mg / kg sodium selenite, the difference was significant (P <0. 05). Conclusions BaP at 125 ~ 500 mg / kg can cause DNA damage in lung cells of mice, while selenite at 0 .75 ~ 3.0 0 mg / kg has obvious effect on lung DNA damage induced by 250 mg / kg BaP Antagonism.