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目的探讨亚砷酸钠(NaAsO_2)对人膀胱上皮细胞系SV-HUC-1细胞内核因子相关因子2(NRF2)活性的影响。方法时间效应实验以4μmol/L NaAsO_2分别处理SV-HUC-1细胞0(对照)、4、8、16、24 h;剂量效应实验设对照组和NaAsO_2处理组(1、2、4、8、10μmol/L);Western blot免疫印迹试验检测NRF2通路相关蛋白(NRF2、NQO1和HO1)表达水平。结果 4μmol/L NaAsO_2处理SV-HUC-1细胞4、8、16、24 h后,与对照组(0.68±0.03)比较,细胞内NRF2蛋白表达水平明显升高,16 h处理组NRF2蛋白表达最高(1.17±0.10),差异有统计学意义(P<0.05);随着染砷剂量增加,NRF2、NQO1和HO1蛋白表达水平增强,与对照组NRF2(0.86±0.05)、NQO1(0.68±0.02)、HO1(0.09±0.01)比较,4和8μmol/L NaAsO_2处理组细胞内NRF2、NQO1、HO1蛋白表达水平分别为(1.12±0.01)和(1.16±0.11)、(0.93±0.01)和(1.15±0.19)、(1.28±0.01)和(1.30±0.02),均明显升高,差异均有统计学意义(均P<0.05)。结论 NaAsO_2急性暴露能够活化NRF2通路,引起膀胱上皮细胞适应性抗氧化反应增强。
Objective To investigate the effect of sodium arsenite (NaAsO_2) on the activity of nuclear factor related factor 2 (NRF2) in human bladder epithelial cell line SV-HUC-1. Methods The effects of time-effect treatment were treated with 4μmol / L NaAsO 2 for 0 (control), 4,8,16,24 h in SV-HUC-1 cells respectively. The dose-response experiments were performed in control and NaAsO 2 treated groups 10μmol / L). Western blot was used to detect the expression of NRF2, NQO1 and HO1. Results After treated with 4μmol / L NaAsO 2 for 4, 8, 16 and 24 hours, the expression of NRF2 protein in SV-HUC-1 cells was significantly increased compared with that in control group (0.68 ± 0.03) (1.17 ± 0.10), the difference was statistically significant (P <0.05). With the increase of arsenic dose, the expression of NRF2, NQO1 and HO1 protein were increased. Compared with the control group, NRF2 (0.86 ± 0.05), NQO1 (0.68 ± 0.02) (1.12 ± 0.01) and (1.16 ± 0.11), (0.93 ± 0.01) and (1.15 ± 0.01), respectively in the groups treated with 4 and 8μmol / L NaAsO_2 0.19), (1.28 ± 0.01) and (1.30 ± 0.02), respectively, which were significantly increased (all P <0.05). Conclusion The acute exposure to NaAsO 2 can activate the NRF2 pathway and cause the adaptive antioxidant response of bladder epithelial cells to be enhanced.