论文部分内容阅读
目的研究双顺反子逆转录病毒载体介导ＩＬ－２基因转导成纤维细胞对小鼠肝癌的治疗作用。方法利用双顺反子逆转录病毒载体ｐＧＣＥＮ／ＩＬ－２将ＩＬ－２基因转导小鼠成纤维细胞ＮＩＨ３Ｔ３，然后将分泌ＩＬ－２的成纤维细胞与６０Ｇｙγ射线照射的肝癌Ｈ２２细胞皮下植入三天前５×１０５或２．２×１０５肝癌Ｈ２２细胞皮下注射的ＢＡＬＢ／ｃ鼠。结果分泌ＩＬ－２的成纤维细胞治疗组能明显增加荷瘤小鼠的生存率（Ｐ＜０．０２５），抑制肿瘤生长（Ｐ＜０．０５），在肿瘤植入第７０天无肿瘤形成的小鼠再次注射１×１０６Ｈ２２细胞仍然不发生肿瘤。５１Ｃｒ释放试验表明来自照射Ｈ２２与分泌ＩＬ－２成纤维细胞混合液注射的小鼠脾细胞与５１Ｃｒ标记的Ｈ２２细胞共培养后，５１Ｃｒ特异释放明显高于对照组（Ｐ＜０．０５）。结论实验结果表明ＩＬ－２基因修饰的成纤维细胞能诱导针对肝癌的特异免疫反应。
Objective To study the effect of bicistronic retroviral vector-mediated IL-2 gene transduction of fibroblasts on hepatoma in mice. METHODS: IL-2 gene was transduced into mouse fibroblast NIH3T3 using bicistronic retroviral vector pGCEN/IL-2. Subcutaneous implantation of fibroblasts secreting IL-2 and H22 cells irradiated with 60 Gy γ-rays was performed. BALB/c mice injected subcutaneously with 5 x 105 or 2.2 x 105 hepatoma H22 cells three days earlier. Results The fibroblasts secreting IL-2 treatment group significantly increased the survival rate of tumor-bearing mice (P<0.025), inhibited tumor growth (P<0.05), and had no tumor formation at the 70th day after tumor implantation. The mice re-injected 1×10 6 H22 cells still did not develop tumors. The 51Cr release assay showed that the specific release of 51Cr was significantly higher in the mouse spleen cells injected with the mixture of irradiated H22 and IL-2 fibroblast-producing cells and 51Cr labeled H22 cells than in the control group (P<0.05). Conclusion The experimental results show that IL-2 gene-modified fibroblasts can induce specific immune responses against liver cancer.