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目的建立远志挥发油成分的气相指纹图谱,研究种植与野生、不同提取方法对远志挥发油成分的影响。方法采用超临界CO2萃取对10批不同产地的远志提取挥发油,用气相色谱仪对挥发油进行分析,建立超临界CO2远志挥发油的气相指纹图谱。结果超临界CO2萃取远志挥发油出油率在0.29%~0.82%之间,高于水蒸气蒸馏法提取率,与水蒸气蒸馏法提取法得到的易挥发油成分基本相同,人工种植较野生远志挥发油成分种类更丰富。结论超临界CO2萃取法提取远志挥发油效率高,为研究远志挥发性成分提供了较好的提取方法。
Objective To establish a gas chromatographic fingerprint of volatile oil from Polygala tenuiflora and to study the effects of planting and wild and different extraction methods on the volatile oil components of Polygala. Methods Supercritical CO2 extraction was used to extract volatile oil from ten batches of Polygala tenuifolia from different habitats. Gas chromatograph was used to analyze the volatile oil. The gas chromatographic fingerprint of volatile oil from Polygala tenuifolia Willd Mill. Was established. Results The extraction rate of volatile oil from Polygala tenuifolia Willd in supercritical CO2 extraction ranged from 0.29% to 0.82%, which was higher than that from steam distillation. The composition of volatile oil from the extraction by steam distillation was basically the same. More variety Conclusion The supercritical CO2 extraction of Polygalaceae Volatile Oil is very efficient and provides a good extraction method for the study of Volatile Components of Polygala.