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目的:研究良性前列腺增生组织中线粒体DNA(mtDNA)控制区的突变情况。方法:取18例良性前列腺增生患者手术切除的增生组织和同一患者的白细胞,经PCR扩增和DNA测序得到DNA控制区序列,再与剑桥标准序列对比。以4名健康成年人前列腺组织为对照。对照无突变检出。结果:良性前列腺增生患者增生组织mtD-NA控制区突变率为83%(15/18),突变频率2.1%(24/1120),全部为点突变。结论:良性前列腺增生细胞线粒体DNA控制区存在高突变率,在良性前列腺增生发病机制中的意义有待进一步研究。
Objective: To study the mutation of mitochondrial DNA (mtDNA) control region in benign prostatic hyperplasia. Methods: 18 cases of benign prostatic hyperplasia patients with surgical removal of the proliferation of leukocytes and the same patient by PCR amplification and DNA sequencing DNA control region sequences, and then compared with the Cambridge standard sequence. Four healthy adults prostate tissue as a control. No mutations were detected in the control. Results: The mutation rate of mtD-NA in benign prostatic hyperplasia was 83% (15/18) and the mutation frequency was 2.1% (24/1120), all of which were point mutations. CONCLUSION: There is a high mutation rate in the control region of benign prostatic hyperplasic cells and its significance in the pathogenesis of benign prostatic hyperplasia needs further study.