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绿巨人（Ｓｐａｔｈｉｐｈｙｌｕｍｋｏｃｈｉ）组培苗继代过程中克服细菌污染的主要方式除常规消毒方法外，可预先在培养基中加入一定浓度的抗菌素，从成本、抑菌效果看，以青霉素钠３００ｍｇ／Ｌ的加入量抑菌效果较好，长期在这种培养基上继代无变异苗出现，且再移入不加抗菌素的培养基中培养也无菌产生。克服玻璃化苗产生的主要方法是：降低培养基中的细胞分裂素浓度，ＢＡ浓度以０．５～２ｍｇ／Ｌ较好，适当提高琼脂的浓度及ｐＨ值有利于苗的健壮发育，并减少玻璃苗的发生率；为了预防玻璃苗的产生，最佳琼脂浓度与ｐＨ值的组合是琼脂０．６％、ｐＨ６．４；琼脂０．７％、ｐＨ５．７～６．０；光照以单方向照射、光强度８００ｌｘ为好，上下两个方向同时光照不好。
In addition to the conventional methods of disinfection, the main ways to overcome bacterial contamination during the subculture of Spathiphylum kochi could be to add antibiotics in a certain concentration to the culture medium in advance. From the perspective of cost and bacteriostasis, taking penicillin 300 mg / L The amount of antibacterial effect is better, long-term in this medium, the generation of non-mutated seedlings appeared, and then moved into the medium without antibiotics also cultured aseptically. The main method to overcome the production of vitrification is to reduce the concentration of cytokinin in the culture medium, BA concentration of 0.5 ~ 2mg / L is better, proper agar concentration and pH are conducive to the robust development of seedlings, and reduce The incidence of glass seedlings; In order to prevent the production of glass seedlings, the best combination of agar concentration and pH is 0.6% agar, pH6.4; agar 0.7%, pH5.7 ~ 6.0; Direction of radiation, light intensity 800lx as well, up and down in both directions at the same time poor illumination.