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本文介绍用体外方法测定取自中国的青蒿素和蒿甲醚对泰国分离的恶性疟原虫的效果。实验用的2个非克隆(K_1和K_(31))以及9个克隆(T_9)分别从泰国的北碧和Mae sod地区分离获得。另外从冈比亚得到1个从非洲分离的非克隆(G_1)作为对照。所有分离的原虫株在试验前均在培养皿内用Trager和Jensen蜡烛缸法进行培养。培养液为RPMI1640,内含100ml/L血清。分离的疟原虫株与青蒿素和蒿甲醚接触的浓度为10~(-7)mol/L到10~(-12)mol/L。置于微量测试板的凹井中,在37℃中培养36～72小时。在24和48小时后更换培养液(含药),并按时取样,作薄膜血片镜检原虫,观察药物完全抑制原虫生长的最低浓度(MIC)。实验表明原虫与药物接触36、48或72小时,其MIC值几乎相似,故本试验中以接触药物36小时作为标准。MIC值随最初的原虫血症而定的,在本次试验中最初原虫血症在0.5～1%范围内。
This article describes the effect of artemisinin and artemether taken from China on the isolation of P. falciparum from Thailand in vitro. Two non-clones (K_1 and K_ (31)) and nine clones (T_9) were isolated from Kanchanaburi and Mae Sod areas in Thailand respectively. In addition, one non-clone (G_1) isolated from Africa was obtained from the Gambia as a control. All isolates of the protozoal were cultured in a Petri dish using the Trager and Jensen candle jar methods prior to testing. Culture medium RPMI1640, containing 100ml / L serum. The isolates of Plasmodium were exposed to artemisinin and artemether at a concentration of 10 -7 mol / L to 10 12 mol / L. Placed in a well of a microtest plate and incubated at 37 ° C for 36-72 hours. After 24 and 48 hours, the culture medium (drug-containing) was changed and sampled on time. The protozoa were examined by membrane biopsy, and the lowest concentration (MIC) of the drug that completely inhibited the growth of protozoa was observed. Experiments show protozoa and drug exposure 36,48 or 72 hours, the MIC value is almost similar, so in this experiment to contact with drugs for 36 hours as a standard. The MIC value is dependent on the initial parasitemia, with initial parasitemia ranging from 0.5 to 1% in this test.