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目的:建立肠炎相关性结直肠癌的小鼠模型。方法:给予小鼠单次小剂量腹腔注射氧化偶氮甲烷(azoxymethane,AOM)联合2%葡聚糖硫酸钠(dextran sodium sulpate,DSS)自由饮用,建立肠炎相关性结直肠癌小鼠模型。对照组小鼠给予PBS腹腔注射后普通饮水。每天观察症状,2%DSS 5 d,普通饮水16 d,此21 d为1个循环,共进行4个循环,结束后,断颈处死小鼠,取脾脏、肠系膜淋巴结、结肠,观察并比较两组小鼠脾脏,肠系膜淋巴结大体改变和结肠HE染色情况。结果:与对照组比较,实验组小鼠脾脏、肠系膜淋巴结明显增大,结肠远端出现肿瘤。HE染色结果显示,实验组发病小鼠结肠黏膜层及黏膜下层出现广泛的炎症细胞浸润,腺体组织结构紊乱、破坏,出现腺管样结构,具有组织异型性。结论:单剂量AOM与DSS联合使用可在短期内诱导小鼠结肠肿瘤的发生。
Objective: To establish a murine model of colitis-associated colorectal cancer. Methods: Mice were given a single dose of Azoxymethane (AOM) combined with dextran sodium sulpate (DSS) to establish a mouse model of colitis-associated colorectal cancer. The control mice were given normal drinking water after intraperitoneal injection of PBS. The symptoms were observed every day, 2% DSS for 5 days and normal drinking water for 16 days. The 21 days was 1 cycle for 4 cycles. At the end of the experiment, mice were sacrificed and their spleens, mesenteric lymph nodes and colon were harvested. Group mice spleen, mesenteric lymph node changes and colon HE staining. Results: Compared with the control group, the spleen and mesenteric lymph nodes in the experimental group were significantly increased, and tumors in the distal colon occurred. The results of HE staining showed that there was extensive infiltration of inflammatory cells in the colon mucosa and submucosa of the mice in the experimental group. The structure of the gland was disorganized and destroyed, and the glandular tube-like structure appeared and had atypia. Conclusion: A single dose of AOM combined with DSS can induce the occurrence of colon cancer in mice in a short time.