论文部分内容阅读
目的通过定量分析血液中的甲胎蛋白及组织因子来判断产科术中回收血液的质量。方法 2014年10月-2015年4月本院妇产科收治的前置胎盘、胎盘早剥及胎盘植入拟行剖宫产患者10例,分别于右颈内静脉穿刺置管即刻(T1)、切皮(T2)、胎盘剥离即刻(T3)、胎盘剥离5 min(T4)及30 min(T5)后各个时间点抽取中心静脉血液,并留取经自体血回收机洗涤后的血液(T6)及白细胞过滤器过滤后血液(T7)。随后对收集的标本用ELISA法对血清甲胎蛋白及血浆组织因子进行定量分析。结果血液中的甲胎蛋白经血液回收机洗涤后显著降低,T1、T2、T3、T4、T5各时刻的血清甲胎蛋白比较差异不具统计学意义(P>0.05),分别与T6、T7时刻的甲胎蛋白比较,差异具统计学意义(P<0.01),T6与T7时刻的甲胎蛋白比较,差异不具统计学意义(P>0.05)。各时刻的血浆组织因子比较差异不具统计学意义(P>0.05)。结论产科术中经自体血回收机回收洗涤的血液,联合使用白细胞过滤器回输体内理论上可行。
Objective To quantitatively analyze the blood levels of alpha-fetoprotein and tissue factor to determine the quality of obstetric recovery of blood. Methods From October 2014 to April 2015, 10 cases of placenta previa, placental abruption and placenta accreta for cesarean section were treated in our department of obstetrics and gynecology. Immediate insertion of right internal jugular vein (T1) (T2), immediately after placental ablation (T3), placental abruption 5 min (T4) and 30 min (T5), blood was taken from the venous blood and the blood was washed by autologous blood collection machine (T6) And leukocyte filter to filter the blood (T7). The collected samples were then analyzed by ELISA for serum alpha-fetoprotein and plasma tissue factor. Results The level of alpha-fetoprotein in the blood was significantly lower after washing with the blood collection machine. There was no significant difference in serum alpha-fetoprotein between T1, T2, T3, T4 and T5 (P> 0.05) (P <0.01). There was no significant difference in alpha-fetoprotein between T6 and T7 (P> 0.05). Plasma tissue factor at each time was not statistically significant difference (P> 0.05). Conclusions Obstetric recovery of blood washed by autologous blood collection machine, the combined use of leukocyte filter back to the body theoretically feasible.