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目的研究单次静滴不同剂量头孢噻利在中国健康受试者的血清杀菌活性(SBA)。方法 12名健康受试者单次静滴不同剂量头孢噻利后,于0.5 h(峰时)和5,8,12 h(谷时)采血,微量肉汤稀释法测定不产酶阴沟肠杆菌、产AmpC酶的阴沟肠杆菌和甲氧西林敏感金黄色葡萄球菌(MSSA)的最低抑菌浓度(MIC)及SBA,计算其达标率。结果头孢噻利对3种致病菌均敏感;单次静滴不同剂量头孢噻利在给药后0.5 h,对3种致病菌的SBA均为>1∶1～1∶64;峰时,对产AmpC酶阴沟肠杆菌的同一时间、不同剂量组间的SBA中位数秩和检验差异有统计学意义(P<0.05),不产酶阴沟肠杆菌和MSSA差异无统计学意义(P>0.05);同一菌种不同剂量的SBA中位数差异均无统计学意义(P>0.05)。峰时SBA≥1∶8百分率,不同剂量组间差异均无统计学意义(P>0.05),且峰时SBA≥1∶8百分率对3种致病菌均达88%以上;谷时及其他时间点SBA≥1∶1的百分率,在不产酶阴沟肠杆菌和MSSA部分差异有统计学意义(P<0.05)。结论头孢噻利不同剂量对临床分离的产AmpC酶和不产酶的阴沟肠杆菌与MSSA均有较强的体内杀菌活性。
Objective To study the serum bactericidal activity (SBA) of ceftibromide in Chinese single-dose intravenous infusion at different doses. Methods Twelve healthy subjects were given single dose of ceftizone intravenously at different doses for 0.5 h (peak time) and 5, 8 and 12 h (valley time), and the micro-broth dilution method was used to detect Enterobacter cloacae , The minimum inhibitory concentration (MIC) and SBA of AmpC-producing Enterobacter cloacae and methicillin-sensitive Staphylococcus aureus (MSSA) were calculated. Results Ceftibufen was sensitive to all three pathogens. The single intravenous infusion of different doses of ceftibrin at 0.5 h after administration showed a SBA of> 1: 1 to 1:64 for all three pathogens, (P <0.05). There was no significant difference in the SBA median rank test between different dose groups (P <0.05), but there was no significant difference between the strains without Enterobacter cloacae and MSSA > 0.05). There was no significant difference in SBA median of the same strain (P> 0.05). Peak SBA ≥ 1: 8 percentage, no significant difference between different dose groups (P> 0.05), and the peak SBA ≥ 1: 8 percentage of the three kinds of pathogenic bacteria reached more than 88%; valley and other The percentage of SBA≥1:1 at the time point was statistically different from that of the non-Enterobacter cloacae and MSSA (P <0.05). Conclusion Different doses of cefotaxime have strong in vivo bactericidal activity against AmpC and non-enzyme-producing Enterobacter cloacae and MSSA clinically isolated.