【摘 要】
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During a 2018 antimicrobial resistance surveillance of Escherichia coli isolates from diarrheal calves in Xinjiang Province,China,an unexpectedly high prevalenc
【机 构】
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College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong 510642,China;
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During a 2018 antimicrobial resistance surveillance of Escherichia coli isolates from diarrheal calves in Xinjiang Province,China,an unexpectedly high prevalence(48.5％)of fosfomycin resistance was observed.This study aimed to reveal the determinants of fosfomycin resistance and the underlying transmission mechanism.Polymerase chain reaction(PCR)screening showed that all fosfomycin-resistant E.coli carried the fosA3 gene.Pulsed-field gel electrophoresis(PFGE)and southern blot hybridization revealed that the 16 fosA3-positive isolates belonged to four different PFGE patterns(i.e.,A,B,C,D).The fosA3 genes of 11 clonally related strains(pattern D)were located on the chromosome,while others were carried by plasmids.Whole-genome and long-read sequencing indicated that the pattern D strains were E.coli O101:H9-ST10,and the pattern C,B,and A strains were O101:H9-ST167,O8:H30-ST1431,and O101:H9 with unknown ST,respectively.Among the pattern C strains,the blaCTX-M-14 gene was co-localized with the fosA3 gene on the F18:A-:B1 plasmids.Interestingly,phylogenetic analysis based on core genome single nucleotide polymorphisms(cgSNPs)showed that the O101:H9-ST10 strains were closely related to a Australian-isolated Chroicocephalus-origin E.coli O101:H9-ST10 strain producing CTX-M-14 and FosA3,with a difference of only 11 SNPs.These results indicate possible international dissemination of the high-risk E.coli clone O101:H9-ST10 by migratory birds.
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