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目的：探讨不同热缺血时间下供心冷保存期间ＡＴＰ的下降程度，以确定不同热缺血时间下供心冷保存的时限。方法：新西兰大白兔１８ 只，随机分成１，２，３ 组（ｎ＝ ６） ，开胸后，取左室前壁心肌测ＡＴＰ，按常规取心方法分别在阻断主动脉后（ 热缺血）０ ｍｉｎ（１ 组） ，５ ｍｉｎ（２ 组） ，１０ ｍｉｎ（３ 组） 取下心脏，保存于４℃的Ｒｉｎｇｅｒ’ｓ 液中，３０ ｍｉｎ，１ ｈ，２ ｈ，３ ｈ，４ ｈ 取左室心肌测ＡＴＰ（ 其中１ 组加测５ ｈ） 。结果：热缺血０ ｍｉｎ，心脏保存于４ ℃Ｒｉｎｇｅｒ’ｓ 液中，ＡＴＰ下降至缺血前５０％ 约为４～５ ｈ，热缺血５ ｍｉｎ，约为３ ｈ，热缺血１０ ｍｉｎ，约为１ ｈ。结论：用４℃的Ｒｉｎｇｅｒ’ｓ 液保存供心，热缺血０ ｍｉｎ，冷存时限为３ ～４ ｈ；热缺血５ ｍｉｎ，冷存时限为２ ｈ；热缺血应小于１０ ｍｉｎ。
OBJECTIVE: To investigate the decrease of ATP during cardiomyocyte storage under different warm ischemia to determine the time frame for cold preservation under different warm ischemia. Methods: Eighteen New Zealand white rabbits were randomly divided into 1, 2 and 3 groups (n = 6). After thoracotomy, the myocardium of the anterior chamber of the left ventricle was measured for ATP. After aortic occlusion Blood samples were collected from Ringer’s solution at 4 ° C for 30 min, 1 h, 2 h, 3 h, 4 h h to take left ventricular myocardial ATP (including 1 group plus test 5 h). Results: During warm ischemia, the hearts were stored in Ringer’s solution at 4 ℃. ATP decreased to about 50% before ischemia, about 4 ~ 5 hours, warm ischemia for 5 minutes, about 3 hours, and warm ischemia for 10 minutes , About 1 h. CONCLUSION: The donor hearts were preserved with Ringer’s solution at 4 ° C for 0 min and the cold storage time was 3-4 h. The warm ischemia was 5 min and the cold storage time was 2 h. The warm ischemia should be less than 10 min.