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目的建立用高效液相色谱分析稻田水、土壤、稻米和小麦粉中哌虫啶残留的方法。方法稻田水用二氯甲烷提取,土壤、稻米和小麦粉用乙腈提取,采用高效液相色谱紫外检测器进行检测。结果在0.5~10mg/L范围内,哌虫啶浓度与色谱峰面积呈良好线性关系,相关系数为0.9998,哌虫啶的最小检出量为1.0 ng。哌虫啶在稻田水、土壤、稻米和小麦粉中的平均添加回收率为78.50%~96.61%,变异系数为2.54%~7.94%。哌虫啶最低检测浓度为0.05 mg/kg。结论本方法准确可靠,操作简便快速,节约溶剂,对环境污染小,符合农药残留分析的要求。
OBJECTIVE To establish a HPLC method for the determination of pipemidine residue in rice paddy water, soil, rice and wheat flour. Methods Paddy water was extracted with dichloromethane, soil, rice and wheat flour were extracted with acetonitrile and detected by high performance liquid chromatography with ultraviolet detector. Results In the range of 0.5 ~ 10mg / L, the concentration of pipermidine showed a good linear relationship with the chromatographic peak area, the correlation coefficient was 0.9998, and the detection limit of piracetam was 1.0 ng. The average recoveries of pipermidine in paddy water, soil, rice and wheat flour ranged from 78.50% to 96.61%, and the coefficients of variation ranged from 2.54% to 7.94%. The lowest detectable concentration of pipemidine is 0.05 mg / kg. Conclusion The method is accurate, reliable, simple, rapid, solvent-saving, less polluting to the environment and meets the requirements of pesticide residue analysis.