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用小麦条锈菌生理小种对中梁93447与感病品种铭贤169的杂交后代F1、F2和F3代进行苗期温室抗条锈性遗传分析,结果表明中梁93447对CYR30的抗病性由1对显性基因控制。用中梁93447×铭贤169 F2代分离群体建立抗、感DNA池,在F2代群体中通过SSR标记技术寻找与抗病基因连锁的分子标记,发现7个位于5BS上的标记Xwmc813、Xwmc740、Xgwm159、Xbarc4、Xwmc616、Xwmc363和Xbarc89与目的基因连锁,遗传距离分别为17、12.9、8.3、4.5、3.7、9.1和20.8cM。系谱分析及分子标记分析表明,该基因可能来自中间偃麦草,暂命名为YrZL93447。与已定位于5B染色体上的抗条锈病基因的比较研究表明,YrZL93447可能是1个新的抗条锈病基因。用SSR引物BARC4和WMC616检测43个黄淮麦区主栽品种,其中7%的黄淮麦区主栽品种具有与YrZL93447基因相同的标记位点。
The genetic analysis of the resistance to stripe rust in the greenhouse of seedlings of F2 generation and F2 generation of Zhongliang 93447 and susceptible cultivar Mingxian 169 was carried out with the wheat stripe rust races. The results showed that the resistance of Zhongliang 93447 to CYR30 Control by a dominant pair of genes. The resistant and susceptible DNA pools were isolated from F2 population of Zhongliang 93447 × Mingxian 169, and the molecular markers linked to disease-resistance genes were searched by SSR markers in F2 population. Seven markers, Xwmc813, Xwmc740, Xgwm159, Xbarc4, Xwmc616, Xwmc363 and Xbarc89 were linked to the target gene with genetic distances of 17, 12.9, 8.3, 4.5, 3.7, 9.1 and 20.8 cM, respectively. Phylogenetic analysis and molecular marker analysis showed that this gene may be derived from Thinopyrum intermedium and temporarily named YrZL93447. A comparative study with stripe rust resistance genes located on the 5B chromosome indicated that YrZL93447 may be a new stripe rust resistance gene. 43 main cultivars of Huanghuai wheat were detected by SSR primers BARC4 and WMC616, of which 7% had the same locus as YrZL93447.