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目的探讨八肽胆囊收缩素(CCK-8)对脂多糖(LPS)诱导的单核细胞RAW264.7细胞内质网应激(ERS)的影响。方法 RAW264.7细胞分为对照组、LPS组、CCK-8组、devazepide组(DEV组)、CCK-8+LPS组及DEV+CCK-8+LPS组,采用RT-PCR法检测X盒结合蛋白1的活性形式(XBP1s)的m RNA表达水平,Western blotting检测ERS标志性蛋白葡萄糖调节蛋白78(GRP78)和C/EBP同源蛋白(CHOP)的蛋白表达水平,ELISA法检测细胞培养上清液中IL-1β水平。结果与对照组相比,LPS组的XBP1s m RNA表达水平、GRP78和CHOP的蛋白表达水平均升高(P<0.05);与LPS组相比,CCK-8+LPS组的XBP1s m RNA表达水平、GRP78和CHOP的蛋白表达水平均降低(P<0.05);与CCK-8+LPS组相比,DEV+CCK-8+LPS组的XBP1sm RNA和GRP78、CHOP蛋白表达水平均升高(P<0.05)。IL-1β含量的变化趋势与CHOP蛋白表达的变化趋势一致。结论 CCK-8通过1受体抑制LPS诱导的ERS,减少IL-1β的生成,从而发挥抗炎作用。
Objective To investigate the effect of octapeptide cholecystokinin (CCK-8) on the endoplasmic reticulum stress (ERS) induced by lipopolysaccharide (LPS) in monocytes RAW264.7 cells. Methods RAW264.7 cells were divided into control group, LPS group, CCK-8 group, devazepide group (DEV group), CCK-8 + LPS group and DEV + CCK-8 + LPS group. The mRNA expression of XBP1s was detected by Western blotting. The expression of GRP78 and CHOP protein were detected by Western blotting. The expression of ERK, Fluid IL-1β levels. Results Compared with the control group, the levels of XBP1s mRNA and the protein expressions of GRP78 and CHOP in LPS group were significantly increased (P <0.05). Compared with LPS group, the expression level of XBP1s mRNA in CCK-8 + LPS group (P <0.05). Compared with CCK-8 + LPS group, the mRNA levels of XBP1smRNA, GRP78 and CHOP in DEV + CCK-8 + LPS group were significantly increased (P < 0.05). The change trend of IL-1β content is consistent with the change trend of CHOP protein expression. Conclusion CCK-8 can inhibit the LPS-induced ERS and reduce the production of IL-1β through 1 receptor, which can exert anti-inflammatory effects.