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目的探讨大鼠腹部开放伤合并海水浸泡对肠道细菌的影响。方法将50只成年健康Wistar大鼠随机分为5组,每组10只。A组为正常对照组,B组为单纯腹部开放伤组,C组为单纯腹部海水浸泡组,D组为腹部开放伤合并生理盐水浸泡组,E组为腹部开放伤合并海水浸泡组。建立大鼠腹部开放伤合并海水浸泡创伤模型,每组大鼠取全血、肝脏和肠系膜淋巴结,分别制备成血浆、肝和肠系膜淋巴结匀浆进行细菌培养并计数菌落数。结果大鼠血浆、肝脏和肠系膜淋巴结匀浆上清液细菌培养结果显示,正常对照组和单纯腹部海水浸泡组均无细菌生长;腹部开放伤合并海水浸泡组均有细菌生长,且菌落数显著高于正常对照组或腹部开放伤合并生理盐水浸泡组(P<0.01)。结论大鼠腹部开放伤合并海水浸泡后肠黏膜屏障功能受损,肠道细菌发生易位。
Objective To investigate the effects of open abdomen injury and seawater immersion on intestinal bacteria in rats. Methods Fifty adult healthy Wistar rats were randomly divided into 5 groups with 10 in each group. Group A was normal control group, group B was simple abdominal open injury group, group C was simple abdominal seawater immersion group, group D was abdominal open injury combined with saline immersion group, group E was open abdominal wound and seawater immersion group. The model of traumatic injury with open abdominal wound and seawater immersion in rats was established. Whole blood, liver and mesenteric lymph nodes were obtained from each group of rats. The plasma, liver and mesentery lymph nodes were prepared for bacterial culture and the number of colonies was counted. Results The results of bacterial culture in the supernatant of plasma, liver and mesenteric lymph node showed that there was no bacterial growth in normal control group and simple abdominal seawater immersion group. Bacteria growth was observed in open abdominal wound and seawater immersion group, and the number of colonies was significantly higher In the normal control group or the abdomen open injury and saline immersion group (P <0.01). Conclusion The rats with open abdomen injury and seawater immersion impaired intestinal barrier function, intestinal bacterial translocation.