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著者曾以感染着X-和Y-病毒的早熟白品种的馬鈴薯块茎,用血清沉淀反应和在千日紅上接种的方法,研究了块茎发芽期間温度、光和氧气对幼芽內X—病毒浓度的影响。在試驗中均用同一块茎的切块作每种条件变化的处理。在定温箱內所做的发芽温度試驗的結果指出,块茎在20℃中发出的幼芽內X-病毒的浓度最高,沉淀滴度一般在1/80—1/160之間,最高的达到1/320,千日紅上平均半叶斑点数目約为30。16°及24℃中的病毒浓度相近,沉淀滴度約为20℃的1/2,斑点数目达20℃的2/3左右。28℃发出的幼芽內病毒浓度最低,平均沉淀滴度約在1/10—1/20之間,平均半叶斑点数目为11.9。块茎在黑暗和光照下发芽的試驗表示,在光照下发出的幼芽內X-病毒浓度显著低于黑暗下发芽的,其沉淀滴度的比例約为1∶4。当块茎在正常空气以及10%、5%的氧气条件下发芽时,沉淀滴度和接种試驗都未发現幼芽內X-病毒浓度的可测定的差異,而在氧分压低的条件下发的芽由于生长量較少,按整芽計算的病毒总量也相应較低。
The authors used potato tubers infected with the precocious white varieties of X- and Y-viruses to study the effect of temperature, light and oxygen on the X-virus concentration in young shoots during tuber germination using a serum sedimentation reaction and inoculation on A. amurensis influences. In the experiment with the same tuber cut for each condition change treatment. The result of the germination temperature test conducted in the thermostat indicated that tuber germinated at 20 ° C had the highest concentration of X-virus and the precipitation titer was generally between 1/80 and 1/16 and the highest reached 1 / 320, the average hemizyme perianth half the number of spots is about 30.16 ° and 24 ° C virus concentration is similar to precipitation titer of about 20 ° C 1/2, the number of spots up to 20 ° C 2/3 or so. The lowest concentration of virus in shoots was 28 ℃, the average sediment titer was about 1/10 to 1/20, and the average number of half leaf spots was 11.9. Experiments in which tubers germinated in darkness and light showed that the X-virus concentration in shoots released under illumination was significantly lower than that in darkness and the ratio of precipitated titers was about 1: 4. When the tubers germinated under normal air and at 10%, 5% oxygen, no measurable difference in X-virus concentration in the shoots was found for both the sedimentation titer and the inoculation tests, but under conditions of low oxygen partial pressure Due to the small amount of growth buds, the total amount of virus calculated according to whole buds is correspondingly lower.