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目的探讨苍术麸炒前后对脾虚大鼠胃肠动力学的影响,并探讨其可能的作用机制。方法将雄性SD大鼠随机分为正常组、模型组、生苍术组(10.0g·kg-1)、麸炒苍术组(10.0g·kg-1)、多潘立酮组(5.0mg·kg-1),共5组,每组10只。除正常组外,其余各组喂饲小承气汤煎剂加饥饱失常建立脾虚证大鼠模型,建模15d。建模结束后,给予相应药物灌胃,正常组及模型组给予等量生理盐水,每天1次,连续10d。10d后所有大鼠均用炭末灌胃法行大鼠胃内残留率、小肠推进比的测定,腹主动脉采血用放射免疫法检测血浆胃动素(MTL)、P物质(SP)、生长抑素(SS)含量,比色法测定大鼠空肠上皮细胞三磷酸腺苷(ATP)的含量,免疫组化法测定大鼠胃窦平滑肌细胞内肌球蛋白轻链激酶(MLCK)及结肠组织中干细胞因子(c-kit)表达量。结果与模型组比较,正常组、生苍术组、麸炒苍术组、多潘立酮组的胃内残留率明显下降、小肠推进比明显升高,而大鼠血浆MTL、SP和SS含量均不同程度升高,差异具有统计学意义(P<0.05,P<0.01)。与模型组比较,生苍术组、麸炒苍术组、多潘立酮组大鼠空肠上皮细胞ATP的含量及胃窦平滑肌细胞内MLCK和结肠组织中c-kit的表达量不同程度升高,差异均有统计学意义(P<0.05,P<0.01);与生苍术组比较,麸炒苍术组上述作用更加明显,差异均有统计学意义(P<0.05,P<0.01)。结论苍术提取物可从不同方面整体改善由于脾虚而导致的大鼠胃肠功能障碍,对因脾虚而导致的胃肠功能紊乱有较好的调节和治疗作用,且麸炒苍术的上述作用优于生苍术。
Objective To investigate the effects of Cortex Atractylodis before and after bran on gastrointestinal motility in rats with spleen deficiency and to explore its possible mechanism. Methods Male Sprague-Dawley rats were randomly divided into three groups: normal group, model group, raw herb group 10.0g · kg-1, 10.0g · kg-1 and 5.0mg · kg- , A total of 5 groups of 10. In addition to the normal group, the rest of the group feeding small Chengqi Decoction plus hungry enough to establish rat model of spleen deficiency syndrome, modeling 15d. After modeling, the corresponding drugs were given intragastrically, and the normal group and model group were given the same amount of normal saline once a day for 10 days. After 10 days, all the rats were treated with charcoal gavage to determine the gastric residual rate, small intestine propulsion ratio, and the abdominal aorta blood samples by radioimmunoassay. Plasma motilin (MTL), substance P (SP) (SS), the contents of ATP in jejunum epithelial cells were determined by colorimetric method. The expressions of SLCs and MLCK in rat gastric antral smooth muscle cells were detected by immunohistochemistry. (c-kit) expression level. Results Compared with the model group, the gastric residual rate in normal group, crude herb group, bran-fried herb group and domperidone group decreased significantly, the small intestine propulsion ratio was significantly increased, while the contents of MTL, SP and SS in plasma were increased to some extent , The difference was statistically significant (P <0.05, P <0.01). Compared with the model group, the content of ATP in jejunum epithelial cells of rats in Atractylodes japonica, Atractylodes maculata group and domperidone group and the expression of c-kit in colon smooth muscle cells in MLCK and colon tissue increased to some extent, with statistical differences (P <0.05, P <0.01). Compared with the raw herb group, the above effects were more obvious in the bran-fried and herb group, the difference was statistically significant (P <0.05, P <0.01). Conclusion The herb extract can improve gastrointestinal dysfunction in rats as a whole due to deficiency of spleen, regulate and treat gastrointestinal dysfunction caused by spleen deficiency, and the above-mentioned effects of bran-herb extraction are superior to Health Atractylodes.