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目的:研究转酮醇酶基因家族在肝细胞癌(hepatocellular carcinoma,HCC)中的表达及其与临床病理特征之间的相关性。方法:检测42例HCC组织及对应癌旁组织中转酮醇酶活性;采用RT-PCR法检测转酮醇酶基因家族各成员[转酮醇酶(transketolase,TKT)基因、转酮醇酶样基因1(transketolase-like1,TKTL1)和转酮醇酶样基因2(transketolase-like2,TKTL2)]mRNA在HCC及癌旁组织中的表达,并研究其与临床病理特征之间的相关性。结果:HCC组织中的转酮醇酶活性明显高于癌旁组织(P<0.05);TKTL1 mRNA的阳性表达率明显高于TKT和TKTL2 mRNA的阳性表达率(P<0.05),HCC中TKTL1 mRNA的表达率及表达量均明显高于癌旁组织(P<0.05),且TKTL1 mRNA的表达同肿瘤分化程度,是否有静脉癌栓,肝内有无卫星灶以及5年生存情况有关(P<0.05)。结论:转酮醇酶在HCC的发生发展中起重要作用,而TKTL1 mRNA的高表达是HCC转酮醇酶活性增高的主要原因。HCC中TKTL1 mRNA的高表达预示着肝癌的分化差,易于转移以及预后不佳。
Objective: To study the correlation between the transketolase gene family and hepatocellular carcinoma (HCC) expression and its clinicopathological features. Methods: The transketolase activity was detected in 42 cases of HCC tissues and corresponding paracancerous tissues; the transketolase (TKT) gene and transketolase-like genes were detected by RT-PCR. Expression of transketolase-like 1 (TKTL1) and transketolase-like 2 (TKTL2) mRNA in HCC and adjacent tissues, and its correlation with clinicopathological features. Results: Transketolase activity in HCC tissues was significantly higher than that in paracancerous tissue (P<0.05). The positive rate of TKTL1 mRNA expression was significantly higher than that of TKT and TKTL2 mRNA (P<0.05). TKTL1 mRNA in HCC The expression rate and expression level of TKTL1 were significantly higher than those in paracancerous tissues (P<0.05), and the expression of TKTL1 mRNA was related to the degree of tumor differentiation, presence of vein tumor thrombus, presence or absence of satellites in the liver, and 5-year survival (P< 0.05). Conclusion: Transketolase plays an important role in the development of HCC, and the high expression of TKTL1 mRNA is the main reason for the increased transketolase activity of HCC. The high expression of TKTL1 mRNA in HCC predicts poor liver cancer differentiation, easy metastasis, and poor prognosis.