论文部分内容阅读
本文报导东亚钳蝎(Buthus martensii Karsch) 毒及用柱层析法从粗毒中纯化的抗癫痫肽(Anti-epilepsy Peptide AEP)对麻醉兔ECG、血压、呼吸和ECOG的作用,以及对离体蟾蜍坐骨神经动作电位的影响。1.对麻醉兔血压、心电、呼吸的影响取1.8～2.5kg兔10只,雌雄兼有,戊巴比妥钠40mg/kg、iv麻醉,用光电RM-46型多导仪监视并记录颈总动脉血压。标Ⅱ导联ECG和呼吸曲线。记录正常曲线15分钟,然后将动物随机平均分为两组。一组iv蝎毒0.5mg/kg。注射后记录上述生理曲线60分钟,待各项指标恢复正常后,再次ⅳ同等剂量蝎毒,并记录生理曲线60分钟。另一组iv抗癲痫肽1.42mg/kg,记录生理曲线60分钟,恢复正常后,用微量注射器向侧脑室注入抗癫痫肽5μg,然后记录生理曲线60分钟。以每15分钟为一个计算单位,结果见表1。
This paper reports the effects of Buthus martensii Karsch and purified anti-epilepsy peptide AEP by column chromatography on the ECG, blood pressure, respiration and ECOG in anesthetized rabbits, Effect of Toad Sciatic Nerve Action Potential. 1. Anesthetic rabbit blood pressure, ECG, respiratory effects take 1.8 ~ 2.5kg rabbit 10, both male and female, sodium pentobarbital 40mg / kg, iv anesthesia, with a photoelectric RM-46-type multi-channel instrument to monitor and record Carotid artery blood pressure. Standard II lead ECG and respiration curve. The normal curve was recorded for 15 minutes, and animals were then randomly divided into two groups. A group iv scorpion venom 0.5mg / kg. After injection of the physiological curve recorded above 60 minutes, until all the indicators returned to normal, again ⅳ the same dose of scorpion venom, and record the physiological curve for 60 minutes. The other group iv iv antiepileptic peptide 1.42mg / kg, recorded physiological curve for 60 minutes, returned to normal, the microinjector was injected into the lateral ventricle anti-epileptic peptide 5μg, and then record the physiological curve for 60 minutes. Take every 15 minutes as a unit of calculation, the result is shown in Table 1.