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通过检测类风湿性关节炎(rheumatoid arthritis,RA)患者外周嗜碱性粒细胞的活化情况,分析其活化与RA发病的关系,进一步阐明RA的发病机制。流式细胞术检测正常对照组和RA患者组外周嗜碱性粒细胞的活化情况及其表达炎症因子和归巢受体的情况。结果显示,RA患者外周嗜碱性粒细胞表达活化标志物CD203c的水平显著高于正常对照组(P<0.05)。RA患者外周表达IL-4、IL-6和IL-13的嗜碱性粒细胞比例以及CD62L和CCR7表达水平均显著高于正常对照组(均P<0.05)。RA患者外周嗜碱性粒细胞处于高度活化状态,并表达高水平的炎症因子和归巢受体,提示其活化后可能趋化至局部,进而参与RA的发病。
By detecting the activation of peripheral basophils in patients with rheumatoid arthritis (RA), the relationship between activation and the pathogenesis of RA was analyzed to further elucidate the pathogenesis of RA. The activation of peripheral basophils and the expression of inflammatory cytokines and homing receptors in normal control group and RA patients were detected by flow cytometry. The results showed that the level of peripheral blood basophil expression marker CD203c in RA patients was significantly higher than that in the normal controls (P <0.05). The proportion of basophils and the expressions of CD62L and CCR7 in peripheral blood of RA patients with IL-4, IL-6 and IL-13 were significantly higher than those of the normal control group (all P <0.05). Peripheral blood basophils in RA patients are highly activated and express high levels of inflammatory cytokines and homeopathic receptors, suggesting that they may chemotaxis to local after activation, which may be involved in the pathogenesis of RA.