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目的建立藿香清胃软胶囊中栀子苷和甘草苷的HPLC含量测定方法。方法用Inertsil C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-水-磷酸(5∶15∶80∶0.1),流速为1.0 m L·min-1,检测该方法的波长为254 nm,柱温为30℃,检测该方法的专属性、标准曲线与定量下限、精密度与回收率和稳定性。结果栀子苷在25.44~254.40μg·m L-1(r=0.999 4)线性关系良好,回收率为96.78%~98.78%。甘草苷在10.24~100.25μg·m L-1(r=0.999 7)线性关系良好,回收率为96.49%~98.09%。结论本方法简便、准确、灵敏度高、重现性好,可作为藿香清胃软胶囊中栀子苷和甘草苷定量控制质量标准。
OBJECTIVE To establish a HPLC method for determination of geniposide and liquiritin in Huoxiang Qingwei Capsule. Methods The mobile phase was methanol-acetonitrile-water-phosphoric acid (5:15:80:0.1) and the flow rate was 1.0 m L · min-1 on an Inertsil C18 column (250 mm × 4.6 mm, 5 μm) The wavelength was 254 nm and the column temperature was 30 ℃. The specificity, standard curve and quantification limit, precision and recovery, and stability of the method were tested. Results Geniposide had a good linearity between 25.44 and 254.40 μg · m L -1 (r = 0.999 4). The recoveries of geniposide ranged from 96.78% to 98.78%. The linear relationship of glycyrrhizin between 10.24 ~ 100.25μg · m L-1 (r = 0.999 7) was good with the recoveries of 96.49% ~ 98.09%. Conclusion The method is simple, accurate, sensitive and reproducible. It can be used as a quality control standard for quantitative determination of geniposide and glycyrrhizin in Huoxiang Qingwei Capsule.