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目的:为开展“肺与大肠相表里”生物学机制研究,初步探索建立肺肠合病的复合模型。方法:实验设空白对照组、肺病组(过敏性哮喘)、肠病组(便秘)和肺肠合病组(过敏性哮喘合便秘)共4组。分别进行肺病(过敏性哮喘)、肠病(便秘)、肺肠合病(过敏性性哮喘合便秘)模型的制备,造模后进行大鼠粪便、哮喘程度及光镜下肺和肠组织形态学观察。结果:肺肠合病组(过敏性哮喘合便秘)既出现了哮喘,又出现了便秘。其粪便重量与肺病组比较减轻,有统计学差异(P<0.05),与肠病组比较亦有减轻,有统计学差异(P<0.01)。其哮喘程度与肺病组比较,程度加重,统计学有差异(P<0.05);其便秘程度与肠病组比较,程度亦加重,统计学有差异(P<0.05)。光镜观察结果显示:肺肠合病组肺组织病理改变较肺病组严重,肺肠合病组的结肠组织病理改变较肠病组严重。结论:肺肠合病组同时出现哮喘和便秘。其哮喘和便秘程度均较肺病组和肠病组严重,初步提示该病理模型的制备方法可行,也似表明“肺”与“大肠”之间可能存在病理互相影响。
OBJECTIVE: To explore the biological mechanism of “lung and colorectal phase”, and to explore the establishment of a compound model of pulmonary bowel disease. Methods: The experiment was divided into 4 groups: blank control group, lung disease group (allergic asthma), enteropathy group (constipation) and pulmonary bowel disease group (allergic asthma combined with constipation). The models of lung disease (allergic asthma), enteropathy (constipation) and pulmonary bowel disease (allergic asthma combined with constipation) were respectively prepared. After the model was established, the stool and asthma were observed and the morphology of lung and intestine under light microscope Learn to observe. Results: Patients with pulmonary bowel disease (allergic asthma and constipation) developed both asthma and constipation. The stool weight and lung disease group were reduced, with statistical difference (P <0.05), and enteropathy group also reduced, with statistical significance (P <0.01). The degree of asthma and lung disease group, the degree of aggravate, statistically significant difference (P <0.05); its degree of constipation and enteropathy group also increased, the difference was statistically significant (P <0.05). The results of light microscope showed that the pathological changes of lung tissue in patients with pulmonary enteropathy were more serious than those in patients with pulmonary disease, and the pathological changes in colon were more serious than those in patients with intestinal enteropathy. Conclusions: Asthma and constipation occur simultaneously in patients with pulmonary enteropathy. The degree of asthma and constipation than the lung disease group and enteropathy group serious, suggesting that the preparation of the pathological model feasible, it seems that “lung ” and “colon ” may exist between the pathological mutual influence.