运用ATP酶、电镜及体视学方法对局部及全身应用GCS豚鼠表皮LC变化进行系统研究。试图阐明GCS皮肤病学药效作用中可能存在的另一机理—LC途径。结果发现表面涂布DA及PA均可显著降低LC密度,下降幅度与药物效能及浓度有关。腹腔注射DAP也引起LC密度明显降低,同时其形态和着色程度也有改变。电镜观察显示LC与IC亚微结构有损伤征象,形态定量与光镜所见一致。 ATPase, electron microscopy and stereological methods were used to systematically study LC changes of GCS guinea pig epidermis locally and systemically. Trying to elucidate another mechanism that may exist in the efficacy of GCS dermatology - the LC pathway. The results showed that the surface coating of DA and PA can significantly reduce the LC density, the decline rate and drug efficacy and concentration. Intraperitoneal injection of DAP also caused a significant decrease in LC density, while its morphology and coloration also changed. Electron microscopy showed that LC and IC sub-micro-structure damage signs, morphology and light microscopy see the same.