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构建了结球甘蓝cDNA文库。文库的宿主菌为E .coliXL1 Blue。对文库的部分特性进行研究 ,滴度为 2× 10 8～ 4× 10 8pfu/mL ,插入片段的大小均在 5 0 0bp以上 ,以 1kb以上的片段占大多数 ,重组体的重组率为 95 % ,用抗病基因片段作探针进行噬菌体原位杂交 ,杂交信号明显。对文库进行了扩增、保存。
A cabbage cDNA library was constructed. The host of the library is E.coliXL1 Blue. The partial characteristics of the library were studied. The titer was 2 × 10 8 ~ 4 × 10 8 pfu / mL. The sizes of the inserted fragments were more than 500 bp. The fragments with more than 1 kb accounted for the majority. The recombinant recombination rate was 95 %, With anti-disease gene fragments as probe for in-situ phage hybridization, hybridization signal was obvious. The library was amplified and saved.