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目的探讨磁性纳米顺铂微粒热化疗抑制卵巢癌skov-3转移相关基因CD44v6、基质金属蛋白酶2(MMP-2)表达的作用。方法取对数生长期的人卵巢癌skov-3细胞株分为阴性对照组、裸药组、纳米药物组、磁热组和磁热化组。处理并体外培养后采用RT-PCR检测各组MMP-2和CD44v6的表达。结果磁热化组CD44v6、MMP-2 mRNA的表达较其余4组有明显降低(P<0.05)。结论在电磁场介导下的磁性纳米顺铂微粒可以显著抑制卵巢癌侵袭转移相关基因CD44v6、MMP-2的表达,可能有抑制卵巢癌的作用。
Objective To investigate the effect of magnetic nano-cisplatin microparticle thermotherapy on the expression of CD44v6 and MMP-2 in ovarian cancer cell line SKOV-3. Methods Human ovarian cancer cell line SKOV-3 in logarithmic growth phase was divided into negative control group, bare drug group, nano-drug group, magnetocaloric group and magneto-thermal group. After treatment and in vitro culture, the expression of MMP-2 and CD44v6 in each group was detected by RT-PCR. Results Compared with the other four groups, the expression of CD44v6 and MMP-2 mRNA in magnetic heating group was significantly decreased (P <0.05). Conclusion Magnetic nano-cisplatin particles can significantly inhibit the expression of CD44v6 and MMP-2, which may be involved in the invasion and metastasis of ovarian cancer, and may inhibit ovarian cancer.