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我们用组织培养的方法,诱导平贝母的不同器官,形成了愈伤组织,鳞茎和植株,为促进平贝母的繁殖速度创造了条件,经过对愈伤组织的初步测定,证实有生物碱反应,而当年新鳞茎低温处理(3-7℃)后进行的组织培养,使进行“二季作”成为可能,同时也为用组织培养的方法进行贝母生物碱的工业化生产提供了重要条件。本试验共分为两个阶段进行的。第一阶段:80年7月18日至81年2月,主要进行鳞片切块培养试验,具体又分为低温(3-7℃,65-70天)及未经低温处理(即收获后
We use tissue culture method to induce different Fritillaria organs, forming callus, bulbs and plants, to promote the Fritillaria propagation speed to create the conditions, after the initial determination of callus, confirmed alkaloids However, the tissue culture performed after the new bulbs cryogenic treatment (3-7 ° C) in the current year made it possible to conduct “two seasons” and also provided the important conditions for industrialized production of the alkaloids of Fritillaria by tissue culture. This test is divided into two stages. The first stage: July 18, 1980 to February 2001, the main scale cut test, the specific is divided into low temperature (3-7 ℃, 65-70 days) and without the low temperature treatment (ie, after harvest