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用生物素－亲合素酶联法（ＢＡＥ）测定正常大鼠肝细胞表面ＨＤＬ受体的特性。实验结果表明，无载脂蛋白Ｅ高密度脂蛋白（ａｐｏＥＨＤＬ）与大鼠肝细胞具有高亲和力、高专一性结合，其平衡解离常数Ｋｄ＝５．５２ｍｇ／Ｌ，最大结合量Ｂ（ｍａｘ）＝１７３μｇ／ｇ细胞蛋白。本法灵敏度高、反应温和，不影响ＨＤＬ与其受体的结合能力。
The characteristics of HDL receptors on the surface of normal rat hepatocytes were determined by biotin-avidin assay (BAE). The results showed that apoEHDL without apolipoprotein E had high affinity and high specific binding to rat hepatocytes. The equilibrium dissociation constant Kd was 5.52 mg / L and the maximum binding capacity B (max) ) = 173 μg / g cellular protein. The law of high sensitivity, mild reaction, does not affect the binding capacity of HDL and its receptors.