我们采用三种方法阻断大脑中动脉(MCA)制备大鼠局灶性脑梗塞模型,并比较它们的优缺点。 材料和方法 采用Wistar大鼠,雌雄各半,体重为310～440 g。0.5％戊巴比妥钠(40mg/kg)腹腔麻醉后,置右侧仰卧位。沿耳眼连线中点弧形切开头皮约2.5cm。在手术显微镜下沿颞骨缘剪开颞肌筋膜(前至眼球后,下达颧弓),剪断颞肌在下颌骨翼突上的止点,并剥离附着在颧弓根的肌肉。以咬骨钳除去颞骨的颧突,充分暴露颞骨。三种方法阻断MCA:①MCA远端结扎:在颧弓根前方颞骨钻 We used three methods to block the middle cerebral artery (MCA) in the preparation of rat focal cerebral infarction model, and to compare their advantages and disadvantages. Materials and methods Wistar rats, male and female, weighing 310 ~ 440 g. 0.5% pentobarbital sodium (40mg / kg) after intraperitoneal anesthesia, set the right supine position. Along the mid-point along the ear ear cut arc about 2.5cm. The temporal fascia (cut to the zygomatic arch before the eyeball) was cut along the temporal bone under a surgical microscope. The temporal muscle was cut off at the stop of the mandibular wing and the muscle attached to the zygomatic arch was dissected. Remove the temporal zygomatic process with a rongeur and fully expose the temporal bone. Three ways to block the MCA: ①MCA distal ligation: temporal bone in front of the zygomatic arch drill