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目的了解2016年北京市某幼儿园一起手足口病聚集性疫情CA6的分子流行病学特征,分析其变异情况。方法用实时荧光PCR对该聚集性疫情标本进行初步病原学鉴定。提取核酸,对其VP1区全长序列进行RT-nPCR扩增和测序。从Genbank中选取不同年代不同地域的CA6参考序列,利用生物信息学软件进行同源性和系统进化分析。结果共扩增到3条CA6 VP1序列(Genbank登录号为KY231153-KY231155),3株CA6与Genbank参考序列在VP1区的核苷酸序列同源性为83%~98%。进化分析表明,该3株CA6与2012年以来我国流行的CA6在同一进化树分支上。结论引起此次聚集性疫情的CA6毒株与2012年以来我国流行的CA6毒株亲缘关系密切,未发生明显变异。
Objective To understand the molecular epidemiological characteristics of CA6 aggregated hand-foot-mouth disease in a kindergarten in Beijing in 2016 and analyze its variation. Methods Preliminary etiological identification of the collected epidemic samples was performed by real-time fluorescence PCR. The nucleic acid was extracted and the full-length VP1 region was amplified by RT-nPCR and sequenced. The reference sequences of CA6 from different regions of China were selected from GenBank, and the homology and phylogenetic analysis were carried out using bioinformatics software. Results A total of 3 CA6 VP1 sequences (GenBank accession number: KY231153-KY231155) were amplified. The nucleotide sequence homology of the three CA6 and Genbank reference sequences in the VP1 region was 83% -98%. Phylogenetic analysis showed that the three strains of CA6 were on the same branch of phylogenetic tree as CA6 that is popular in our country since 2012. Conclusion The causative CA6 strain caused by this cluster epidemic has a close relationship with the CA6 strain endemic to China since 2012, with no obvious variation.