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从‘霞晖8号’桃中克隆了一个定位于第4条染色体上的钾转运体基因PpeKUP5,该基因编码625个氨基酸,具有10个跨膜结构域;PpeKUP5主要在根部表达,其次是叶和茎中,花和果实中的表达量极低;ABA、重金属Cr和Zn处理均显著诱导其在桃幼苗各组织中的表达,其中Cr处理最为显著,高钾胁迫及重金属Cu处理显著降低了其在根部的表达水平;细菌互补试验表明PpeKUP5具有吸收外界K~+(KCl或K_2SO_4)的功能,且在偏中性pH值条件下最为显著。本研究表明PpeKUP5是一个主导桃树根部K~+吸收的钾转运体,并可能在桃树适应高钾及重金属Cr、Zn和Cu等胁迫处理和ABA响应中起着重要作用。
PpeKUP5, a potassium transporter gene located on chromosome 4, was cloned from ’Xia Hui 8’. It encodes 625 amino acids and possesses 10 transmembrane domains. PpeKUP5 is mainly expressed in the roots, followed by leaves And stems, flowers and fruits. The expression of ABA, heavy metals Cr and Zn in all tissues of peach seedlings was significantly induced, among them, the treatment with Cr was the most significant. The treatment with high potassium stress and heavy metal Cu significantly decreased The expression level of PpeKUP5 in the roots was detected. The results of bacterial complementation tests showed that PpeKUP5 has the function of absorbing K + (KCl or K 2 SO 4) from the outside and most notably under the neutral pH condition. This study showed that PpeKUP5 is a potassium transporter that predominates K + uptake in peach roots and may play an important role in peach adaptation to stress treatments such as high potassium and heavy metals Cr, Zn and Cu, and ABA responses.