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用电镜观察了在0℃贮藏4个月和在20℃室温发病后红星苹果(Mains p1L’,llLtl ill。)阴阳面果皮的超微结构。发现苹果果实角质层中电子密集物与虎皮病的发生有密切关系。该电子密集物可由角质层沿细胞壁向内层细胞移动,在接触到细胞时细胞质膜解体,内部结构破坏,并可进人细胞内部,增大细胞的电子密度。推测该电子密集物可能是a一法尼烯氧化反应后的混合物。冷藏结束时,果实阴阳面均无虎皮病发生。果实阳面细胞内部结构较好,内层细胞胞壁电子密集物少,而果实阴面有的表皮细胞已解体,内层细胞胞壁电子密集物多。在果实阴面发病后,阳面仍未发病。果实阳面细胞内部结构仍较好,只少数下皮外层细胞被破坏,而果实阴面果皮细胞已解体,电子密度增加。研究认为,角质层中电子密集物可从表皮、下皮细胞外部伤害细胞。
The ultrastructure of the pericarp of the yin-yang apple (Mains p1L ’, llLtl ill.) After 4 months of storage at 0 ℃ and room temperature of 20 ℃ were observed by electron microscope. It is found that there is a close relationship between the electron density in the stratum corneum of apple fruit and the occurrence of tiger skin disease. The electron dense material can move along the cell wall toward the inner layer cell by the stratum corneum, and disintegrate the cell plasma membrane upon contact with the cell, thereby destroying the internal structure and entering the cell interior to increase the electron density of the cell. It is speculated that the electron dense may be a-farnesene oxidation reaction mixture. At the end of refrigerated, there was no tiger skin disease on the yam and yang side of the fruit. The internal structure of the fruit surface cells is better, the inner cell wall electron dense less, and some negative fruit epidermal cells have been disintegrated, the inner cell wall electron dense and more. After the fruit in the shade, the sun has not yet onset. The internal structure of the fruit surface cells is still good, only a small number of outer layer of cells destroyed, while the fruit of the perineal cells have been disintegrated, the electron density increased. Studies suggest that the horny layer of electron dense material from the epidermis, the outer skin damage cells.